Expression and role of Pim1 in cultured cortical neurons with oxygen-glucose deprivation/reoxygen injury / 中国当代儿科杂志

OBJECTIVE@#To study the expression and effect of Pim1 in primary cortical neurons after hypoxic-ischemic injury.@*METHODS@#Cortical neurons were isolated from 1-day-old C57BL/6 mice and cultured in neurobasal medium. On the 8th day of neuron culture, cells were subjected to oxygen-glucose deprivation/reoxygen (OGD/R) treatment to mimic in vivo hypoxic injury of neurons. Briefly, medium were changed to DMEM medium, and cells were cultured in 1% O for 3 hours and then changed back to normal medium and conditions. Cells were collected at 0 hour, 6 hours, 12 hours and 24 hours after OGD/R. Primary neurons were transfected with Pim1 overexpression plasmid or mock plasmid, and then were exposed to normal conditions or OGD/R treatment. They were named as Pim1 group, control group, OGD/R group and OGD/R+Pim1 group respectively. Real-time PCR was used to detect Pim1 mRNA expression. Western blot was used to detect the protein expression of Pim1 and apoptotic related protein cleaved caspase 3 (CC3). TUNEL staining was used to detect cell apoptosis.@*RESULTS@#Real-time PCR and Western blot results showed that Pim1 mRNA and protein were significantly decreased in neurons after OGD/R. They began to decrease at 0 hour after OGD/R, reached to the lowest at 12 hours after OGD/R, and remained at a lower level at 24 hours after OGD/R (P<0.01). Overexpression of Pim1 significantly upregulated the protein level of Pim1. Under OGD/R conditions, the CC3 expression and the apoptosis rate in cells of the Pim1 group were significantly lower than in un-transfected cells (P<0.01).@*CONCLUSIONS@#Hypoxic-ischemic injury may decrease Pim1 expression in neurons. Overexpressed Pim1 may inhibit apoptosis induced by OGD/R.

Research on the Effect of Information Intelligence Technology in Hospital Operation Decision / 中国卫生经济

Objective:To observe and analyze the effects of information intelligence technology in hospital operation decision.Methods:Relevant factors have been analyzed vertically before and after the application of information intelligence technology in Beijing Cancer Hospital.In this case,data should be analyzed before and after the usage of the information intelligence technology,in terms of medical quality (bed usage,average length of hospitalization,the number of cases,etc.),workloads (number of visits,number of discharged patients,etc.),social benefits (patient satisfaction and employee satisfaction),and economic benefits (total assets,net assets,fixed assets,business income,per capita business income,etc.).Results:Mter the implementation of information intelligence technology,bed occupancy rate and the number of discharged was higher than that before implementation,as well as average length of hospitalization was significantly lower than before.Secondly,the number of visits,discharged persons,total assets,net assets,business income and per capita business income and the satisfaction rate of patients and staff after the implementation of the information intelligence technology were significantly higher than those before.Conclusion:Information intelligence technology could improve medical process and service efficiency,as well as reduce medical disputes and doctor-patient contradiction in hospital operation decision-making.Moreover,the satisfaction of doctors and patients could be ameliorated,manpower and material resources could be saved significantly as well,and the management efficiency would be effectively improved.

Risk factors of post-asphyxial multiple organ dysfunction in neonates / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To investigate the risk factors related to post-asphyxial multiple organ dysfunction (PA-MOD) in neonates.</p><p><b>METHODS</b>A total of 397 neonates with birth asphyxia were enrolled from January 2009 to December 2010.The patients were divided into PA-MOD group (n=179) and non-PA-MOD group (n=218). The risk factors of PA-MOD were retrospectively studied.</p><p><b>RESULTS</b>Multivariate logistic regression analysis showed that severe asphyxia, fetal distress, abnormal labor, and decreased amniotic fluid were the risk factors for PA-MOD among the neonates. Spearman rank correlation analysis showed that the number of the involved organs increased along with the increase of age at admission (P<0.05) and with the decrease of gestational age and birth weight (P<0.05).</p><p><b>CONCLUSIONS</b>The efforts should be made to enhance perinatal care for neonates, especially for preterm infants and low-birh-weight infants, to decrease the incidence of MOD.</p>

Relationship between the expression of uncoupling protein 2 and the damage by oxygen free radicals in acute liver failure rats / 中华肝脏病杂志

To investigate the relationship between uncoupling protein 2 (UCP2) expression and the damage caused by oxygen free radicals in acute liver failure rat models. Thirty-five male Sprague-Dawley rats were randomly divided into two groups: the control group (15 rats) and liver failure group (20 rats). The rats were injected intraperitoneally with thioacetamide (TAA) to induce models of acute liver failure. The levels of endotoxin (ET) were detected by double antibody sandwich enzyme-linked immunosorbent assay. The expression of liver UCP2 mRNA was detected by reverse transcription polymerase chain reaction. The superoxide dismutase (SOD) and malonaldehyde (MDA) were detected by spectrophotometry. The expression of UCP2 protein was observed by immunohistochemistry. The data of the two groups were compared using Mann-Whitney U test or ANOVA. The expression of UCP2 mRNA in liver failure group was higher as compared to the control group (P value is less than 0.01); the level of MDA and endotoxin of liver failure group were higher than that of the control group (P value is less than 0.01). SOD of the liver failure group was lower (P value is less than 0.01). There was a certain correlation between UCP2 mRNA expression and ET, SOD and MDA (r = 0.952, -0.667, 0. 634 respectively, P value is less than 0.05 or 0.01). UCP2 is highly expressed in the livers of liver failure rats. A certain correlation perhaps existed between the expression of UCP2 mRNA and the serous SOD, MDA and ET.

Optimizing extraction process of Scutellaria barbata by golden section method / 中国中药杂志

<p><b>OBJECTIVE</b>To probe into the feasibility of golden section method in the optimization of the extraction process of Scutellaria barbata.</p><p><b>METHOD</b>With the determination of the content of scutellarin and total flavonoids, selecting the range of alcohol concentration in the extraction of S. barbata was determined by the golden section method and method of equal interval, and validated it by orthogonal design.</p><p><b>RESULT</b>The best extraction technique is that extract 3 times with 15 times of the alcohol which concentration is 70%, and each time for 1 h.</p><p><b>CONCLUSION</b>Applying golden section method in the extraction of S. barbata is practical, which as a method of preliminary trial, indicates that golden section method can also be applied into the field of optimization of extraction process of traditional Chinese medicine.</p>

GFP gene transfection of dendritic cells mediated by recombinated adeno-associated virus / 中南大学学报(医学版)

OBJECTIVE@#To investigate the transfection condition of Type 2 recombinant adeno-associated virus ( rAAV2) in human dendritic cells(DCs) which were induced from the bone marrow CD34+ hematopoietic stem /progenitor cells.@*METHODS@#CD34+ hematopoietic stem /progenitor cells were purified from the bone marrow mononuclear cells by immunomagnetic beads, and the cells were cultured with IL-4 and GM-CSF and maturated by TNF-alpha on the 5th day. The rAAV2 /GFP was transfected into the induced cells at different time.The DCs were identified by electronic microscope. The expression of GFP was evaluated by flow cytometry and fluorescence microscope.@*RESULTS@#The DCs were induced successfully. The typical morphologic characteristics of DCs were observed under the light microscope and transmission electronic microscope, and the typical phenotypes of DCs could be detected by flow cytometry. The expression rate of GFP gene on the 3rd day, the 5th day and after adding TNF-alpha was 0.45%, 13.54%, and 0.25%, respectively.@*CONCLUSION@#DCs can be induced from the human bone marrow CD34+ hematopoietic stem /progenitor cells, and infected with the rAAV2 /GFP successfully. The longer the induction time of DCs, the higher the transfection efficiency of DC. The transfection efficiency of immature DC is higher than that of mature DC.

Tyroservatide inhibits the growth of human hepatocarcinoma in nude mice / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To investigate the inhibitory effects of tyroservatide and its amino acid mixture on growth of hepatocarcinoma.</p><p><b>METHODS</b>Hepatocarcinoma in nude mice was induced by implantation of cells of human hepatocarcinoma cell line BEL-7402. The inhibition of hepatocarcinoma growth was determined by calculating the tumor volume and measuring the tumor weight. The effects of tyroservatide on tumor cells in nude mice were assessed by immunohistochemical staining of proliferating cell nuclear antigen (PCNA), electron microscopic observation of ultrastructure, and apoptosis of tumor cells using terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL).</p><p><b>RESULTS</b>Tyroservatide significantly inhibited the growth of human hepatocarcinoma in nude mice, with an inhibiting rate more than 60%. But the mixture of amino acid did not show a significant inhibitory effect on the tumor growth. Tyroservatide also induced apoptosis of tumor cells and decreased the expression of PCNA in tumor cells.</p><p><b>CONCLUSION</b>Tyroservatide may significantly inhibit the growth of human hepatocarcinoma in nude mice by inducing apoptosis and inhibiting proliferation of tumor cells.</p>

Effects of glucocorticoids on T helper cells balance in oral lichen planus / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the effects of dexamethasone on Th1/Th2 cytokines in oral lichen planus.</p><p><b>METHODS</b>Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation from OLP patients and healthy controls. PBMC from patients with OLP were stimulated with phytohemagglutinin (PHA) and dexamethasone respectively for 72 h. The concentrations of interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) in culture supernatants were determined by ELISA. The mRNA levels for IFN-gamma and IL-4 in culture cells were evaluated using semi-quantitative reverse transcription-polymerase chain reaction.</p><p><b>RESULTS</b>Compared with healthy controls, the levels of IFN-gamma in OLP patients were significantly lower (P < 0.05). The levels of IL-4 were higher, but not statistically significant (P > 0.05). The ratios of IFN-gamma/IL-4 were lower in patients with OLP (P < 0.05). Dexamethasone significantly inhibited the levels of IFN-gamma and IL-4 (P < 0.01). Moreover, IFN-gamma was inhibited significantly more than IL-4. The levels of IFN-gamma and IL-4 mRNA expression in culture cells were consistent with protein production in supernatants.</p><p><b>CONCLUSIONS</b>Th2 immune response is predominant in OLP. Dexamethasone is an immunosuppressant inhibiting Th1/Th2 cytokines.</p>

Relationship between impaired glucose regulation and intra- or extra-cranial artery occlusive disease / 中华神经科杂志

Objective To observe the prevalence of impaired glucose regulation (IGR) in ischemic stroke patients with or without inter-or extra-cranial arteries occlusive disease,and to analyze the relationship of IGR with large arteries occlusive disease.Methods lschemic stroke patients without diabetes history and with FPG